Composite

Part:BBa_K395998:Design

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-19)


Wolf coli system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1765
    Illegal NheI site found at 1788
    Illegal NheI site found at 18920
    Illegal NheI site found at 18976
    Illegal NotI site found at 18821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
    Illegal BglII site found at 4076
    Illegal BglII site found at 10537
    Illegal BglII site found at 17098
    Illegal BglII site found at 17350
    Illegal BamHI site found at 13322
    Illegal BamHI site found at 18790
    Illegal XhoI site found at 2183
    Illegal XhoI site found at 18830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal NgoMIV site found at 12858
    Illegal NgoMIV site found at 12988
    Illegal AgeI site found at 10099
    Illegal AgeI site found at 12073
    Illegal AgeI site found at 17360
    Illegal AgeI site found at 18574
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 10596
    Illegal BsaI.rc site found at 17181
    Illegal SapI site found at 17008
    Illegal SapI site found at 17020


Design Notes

test


Source

test

References